Proteome analysis is the quantitative determination of proteins in a cell, an organism, or in a complex body fluid at a specific time and under defined conditions. In contrast to the rather static genome, the proteome (= the entity of all proteins in the human body) is dynamic and can therefore change in its qualitative and quantitative composition due to changing conditions (environmental factors, temperature, drug delivery etc.).
The difference between a caterpillar and a butterfly shows this very clearly. Both have the same genome, but their appearance is completely different due to the different composition of their proteins. For this reason, the analysis of the proteome is ideal for mapping the current state of an organism.
Proteins play an important role in the human body. Understanding their meaning and function is the greatest challenge in clinical research. Newly formed or altered proteins can be signs of disease. Such disease-specific proteins are called biomarkers.
As a so-called risk indicator or predictive biomarker, disease-specific biomarkers provide information on whether a disease is imminent, whether the disease already exists (diagnostic biomarker), or how a disease is likely to develop in individual cases (prognostic biomarker).
With the DiaPat® technology as a patented, leading method of proteome analysis, several thousand proteins can be analyzed simultaneously and recognized as (new) biomarkers.
For example, the DiaPat® technology uses 273 proteins for the detection of chronic kidney disease - current diagnostics only consider one protein, albumin. This illustrates the epochal progress: to recognize and differentiate diseases very precisely. For the first time, this accuracy also enables very early detection of diseases. As a result, we can quickly see whether a therapy that has been applied to a patient is effective.
For analysis, a method consisting of capillary electrophoresis coupled with mass spectrometry (CE-MS) is used. The analysis procedure itself is described in detail in a number of scientific publications and complies with the worldwide standards for clinical proteome analysis.
More details, explained in a simple manner, can be found at: Proteome Analysis Technology.